裴勇俊古装新剧9月开播 官方海报首曝光(图)
【Abstract】 Objective To explore the possibility of transferring B gene for the treatment of thods B cDNA was obtained by reverse transcription polymerase chain reaction and recombined to retroviral vector pLXSN. Calcium phosphate-DNA precipitation method was used to transfer PLB SN into packaging cells PA317. Human leukemia cells K 562 and HL-60 were infected with higher titer virus. B protein expression was confirmed by FACS technique. After PBMCs from healthy donor were cultured with B+ K 562 or B+ HL60, 3 HTdR incorporation and IL-2 and IFN-γ expression were determined. Results A significantly increased 3HTdR incorporation in B+ leukemia cell groups was observed (3 680 cpm in K 562 and 3 980 cpm in HL60, respectively) as compared with those in B7 leukemia cell groups (645 cpm in neo-K 562, 866 cpm in Wild-K 562 and 486 cpm in neo-HL-60, 742 cpm in Wild-HL-60). Interleukin-2 and Interferon-γ expressions were found in PBMC cultured with B transfected K 562 and HL-60 cell lines, but not found in those cultured with wild or neo-cell nclusion B transfected K 562 and HL-60 cell lines could induce the proliferation and differentiation of the T cells. It is likely that B transfected cells may be capable of inducing the activity of cytotoxic T lymphocyte, and that B transgene therapy is possible for the treatment of leukemia in childhood.
这名负责人同时还分析，郑州市的合村并城和城中村改造工作也在如火如荼进行中，大批在建房屋将被推向市场，再加上新建普通商品房的入市，郑州的房价、租赁价格到明、后年肯定要面对残酷的竞争，大幅上涨的空间基本不存在。